To study the impression of a number of constraints on mutational tolerance during variety, we selected E. coli dihydrofolate reductase (DHFR) as a product program. DHFR is An important enzyme in folate metabolism that lowers dihydrofolate to tetrahydrofolate and is also essential for thymidine generation. making use of this exercise as The premise for an in vivo choice assay (Reynolds et al., 2011), we aimed initial to measure a mutational landscape for DHFR after which you can to determine how a modify on the mobile setting could have an affect on the landscape. for the reason that DHFR is known to development via a number of conformational states through catalysis (Boehr et al.
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(2020) Altered expression of a quality control protease in E. coli reshapes the in vivo mutational landscape of a product enzyme
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